Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
GFI1

Cell type

Cell type Class
Blood
Cell type
CCRF-CEM
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic

Attributes by original data submitter

Sample

source_name
GFI1_CHIP_rep2
cell lines
CCRF-CEM-GFI1-3XFLAG
chip antibody
Flag (Sigma Aldrich, F1804)
treatment
treated with Doxycycline for 24 hours

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was pruified from sonicated cell lysate (input) or subquentially anti-FLAG antibody precipitation using Zymo ChIP DNA clean & concentrator (Zymo Research) Libriaries were prepared for sequecing acordding to the instructions of NEBNext ChIP-Seq Library Prep Reagent Set

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
47856196
Reads aligned (%)
99.0
Duplicates removed (%)
3.1
Number of peaks
9591 (qval < 1E-05)

hg19

Number of total reads
47856196
Reads aligned (%)
98.2
Duplicates removed (%)
4.5
Number of peaks
9494 (qval < 1E-05)

Base call quality data from DBCLS SRA